FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856305
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG9750.017.5894585
TAGGTAG3900.016.1535855
TCTAGAC4600.015.2171453
GTATTAG7550.014.8341981
TCTATAC8650.014.5662373
ACCCGCG1450.00474186814.48252533
TATACAG10500.014.3331025
CCGGACG1500.00593768613.99977412
CCTACAC3555.986294E-913.8025933
ACTATGC7450.013.623948
GTATAAG8000.013.562281
CTGAGCG2354.5694975E-513.4040379
GTATATA19150.013.3418211
GCGTAGC1600.00909979813.1247879
TACACAG6500.012.9228685
TCCTATA6800.012.8674392
ACAGTCC3558.8961315E-812.8166948
ATTATAC14550.012.7489363
ATACTGA11000.012.7270676
CTATACT8350.012.5746474
Produced by FastQC (version 0.11.5)