FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811153
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAC1503.161459E-416.33261143
ACCGCGC1400.003755447214.9997528
TCCGCAC3302.1336746E-914.8515264
CGCACCT3302.1336746E-914.8515266
TTAGACT6850.014.8168634
CCGCACC3352.6375346E-914.62985565
GTATATA20050.013.7899141
CTAATAC9250.013.621023
GTATAGA9800.013.5708291
TAGACAG5750.013.3907135
GTGTATA6800.013.3817621
GTATATG12650.013.28004551
ACGGTGT4851.8189894E-1112.9894758
TATACAG12150.012.6743375
TATACGG6650.012.6310215
CTTAGAC5357.2759576E-1212.4293583
GTATTAG8200.012.3775021
CCCTGCG2000.002783398812.24945914
TAATACT14350.011.9506914
ACTAGGC2952.8945838E-511.8638833
Produced by FastQC (version 0.11.5)