FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723916
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4450.018.8758035
CGCACCT2552.1729647E-816.47388566
GTATTAG7250.015.9305271
TATACTG10000.014.3495435
GTATATA18350.013.73253251
TATACCG3851.2769306E-913.6359295
TAGGCCG1550.007377940713.5479555
TATACAG12050.013.3605695
CCTGTCG2102.6538668E-413.3329088
ACGGTGT6050.013.3053628
AGCCCGT2405.5683005E-513.1245816
CATATAC10550.012.9379773
TATACGG6000.012.8329245
GTAGGAC2751.4084413E-512.7268673
GTGTATA6150.012.5199261
TATATAC21950.012.4369613
TTATAGT10550.012.2744914
GAGGTCC2305.718188E-412.1735259
GTATAGA8850.011.8640281
ATTATAC11550.011.8178043
Produced by FastQC (version 0.11.5)