FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791427
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA2108.367351E-1121.66606156
CCTACAC3450.019.2748243
CGCGCGG2053.2456228E-818.77996454
TCCTTGC10950.017.5803998
AACGCGC2401.0310032E-817.49951252
CACCCCG1653.3965887E-516.970177
CCTCCTA2401.9627623E-716.0412181
GCCCGGC2051.2601578E-515.36542517
CCCTAGG1605.1652174E-415.3120732
GGCGCGA1859.04098E-515.13471238
TAGGACG1400.003755709414.999584
CTATCCC4450.014.9434044
TCCTATA6800.014.9260542
CCCGCTT4251.8189894E-1214.82435728
GCGCGAG2151.9771811E-514.65075539
GTATCCT5500.014.6359561
CCTTGCG5750.014.6091039
GTAGGAC1708.1799785E-414.4113633
CGGCGCG1951.4153804E-414.35857437
CGCACCT3453.9981387E-914.20448566
Produced by FastQC (version 0.11.5)