FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910777
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3500.019.00089564
CCGCACC3350.018.80685465
CCTAGAC4100.016.2194293
CTAGGAC3251.724402E-915.0768443
CGCACCT4009.094947E-1214.87570166
CATATAC13650.014.8717173
TAGGACC4051.0913936E-1114.691284
TCGCCCG1450.004741592314.48268224
GTATTAG8600.014.2441121
TATACTG13250.013.99992755
ACTGCGC2001.7547657E-413.9999278
TATATAC24800.013.8305733
GTATAGG5400.013.611041
TACACAG6500.013.4614685
CTATACA10150.013.4482064
TATACAG12300.013.3739145
TAGGTAG4455.820766E-1113.3707175
TACTCTG5800.013.2757927
CTAATAC9350.013.1015353
TTATACT16300.013.0980924
Produced by FastQC (version 0.11.5)