FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767976
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC5000.019.60304564
CCGCACC5700.016.58152465
CGCACCT5500.016.54802566
ACTATGC11650.015.6222058
CTATGCA11050.014.8867719
CCGCGCG1708.1784447E-414.41218128
TAATACT21650.014.3876274
CTAATAC12850.014.1630663
ACCGCGC2505.270138E-613.9998988
GGACCGC2001.7555135E-413.9996466
TACACCG5800.013.878965
TATACAG15750.013.7774295
ACCGCTC3701.0708391E-813.2431488
TAGACGG4902.1827873E-1112.8568185
CTAGGAC4103.272362E-912.8045553
GTATATA25900.012.7023821
GTATATG15850.012.5864321
GTATTAG10650.012.48794651
TATACCG6800.012.3526295
TATACTG16250.012.2766125
Produced by FastQC (version 0.11.5)