FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717950
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2650.025.09350854
CGCGCCG1801.7055754E-719.44380215
CGCGGGA3300.018.02970756
GCGCGGG3500.017.99940355
CCTCCTA3057.021299E-1016.065041
CGTCCGG3201.3860699E-915.31283832
GTCCTAA5650.014.8667651
AACGCGC4251.8189894E-1214.82303952
ACTATGC11400.014.73689658
GCTCCTA3454.0090526E-914.2024291
TCCTTGC12100.013.5950928
ACGCGCG4404.7293724E-1113.5222853
TCCTCAC9350.013.4759868
CCTACAC4506.91216E-1113.2217853
CTATGCA8850.013.0508959
CTAATAC11550.013.0298723
TAGGACG2203.932077E-412.7273212
CGGCGCG3305.1077404E-712.72685138
CGGGAAC4952.5465852E-1112.72685158
TACCTCC7450.012.6841444
Produced by FastQC (version 0.11.5)