FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024720
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG1903.0095362E-718.42123433
CGCACCT3600.017.50146966
CCTACAC3650.017.260023
CCGCACC3255.456968E-1217.23221665
GTATTAG8750.015.9997621
TCCGCAC3301.2551027E-1015.91042664
ACAGTCC3151.1077645E-915.555718
CGGTCGC1350.002947447815.55570911
CCGGTCG1656.522678E-414.84863110
CGCCGAG1656.5238995E-414.84826520
GTCTATA5900.014.8302891
GTATAGA10100.014.5542391
CCGCCCG2454.27319E-614.28585510
TATACGG6000.013.9997925
CTAGGAC2759.171308E-713.9997923
CCGCGCT2303.7334463E-513.69544935
CTAGACT3354.1663952E-813.5818884
CGGTCCG1550.007376486413.54852110
CTCCCGC2607.902565E-613.46133921
TCCTAAG5200.013.4613392
Produced by FastQC (version 0.11.5)