FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250800
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCG1650.029.70635261
AGCTCGC1951.8189894E-1225.127160
CTCGCGT2351.6370905E-1120.8576562
CAGCTCG2402.3646862E-1120.41576859
GTGCGCA2453.092282E-1119.9991254
TGTGCGC2653.6379788E-1219.8104553
AGGTGCG2900.019.31103927
TATACAG9800.019.2848665
GCGCAGC2555.4569682E-1119.21484256
CGCGTTG2801.0913936E-1118.75592264
GCGACGT2851.2732926E-1118.42171731
GTGCGAC2851.2732926E-1118.42171729
CACGCAG3300.018.0295116
TAGGGCG1000.00969328717.4992315
ACCACGC2207.277595E-817.499238
TACAGGC3450.017.2456197
GCGTTGC3307.2759576E-1216.97505865
CGCAGCT3104.9112714E-1116.93473857
GGTGCGA2903.4197E-1016.8971628
GTATAAG5400.016.851111
Produced by FastQC (version 0.11.5)