FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178467
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC2001.0677468E-921.00374465
TCCGCAC2203.4942786E-919.09431364
CGCACCT2203.4942786E-919.09431366
GCGCGTC950.007212119618.4204336
TTAGGGT3300.018.0296924
TACACCG2207.275776E-817.4994075
CTTAGGG1452.4400195E-416.8959773
CTGAGCG1554.0549942E-415.8065859
GTGGGCA2101.5814912E-515.0001289
TGCGTGC1656.5198814E-414.8486128
GTGTAGG2151.9761575E-514.6506651
TAGACAG3604.656613E-1014.5828395
GCCGCGT1450.00474106714.48226646
ATACTAT11900.014.4112756
CCCGGAT2951.30216E-714.23680524
CTATGCA5200.014.1347349
CTAATAC5950.014.1171673
ACAGTGG4505.456968E-1214.0001198
GGTAGTC3252.7943315E-814.0001197
GGTGCGT1500.005935020314.00011710
Produced by FastQC (version 0.11.5)