FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120640
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG657.9614914E-426.9217573
CGCACCT1656.783739E-821.21675966
TCCGCAC1709.298674E-820.59273764
CTAGGAC1558.752959E-720.3215833
CGGCTCA1107.477185E-419.0908249
GACCCTC950.007210679418.420977
GTATTAG4800.016.770011
GTATAAC4800.016.770011
CCGCACC2108.562329E-716.67031165
CTTACAC2753.079549E-916.5446413
TAGGGGT1300.002291516.1530534
CTAGCAC1957.838917E-616.1530533
TAGGTAG2401.9613071E-716.040885
TAGGACC2401.9613071E-716.040884
CTATACT5300.015.1879344
ACAGGGC1400.003753953614.9999348
GTGCGCA2151.9762689E-514.65044454
TACAGGC2901.0560143E-714.4826957
AAGCGGG1450.004741348314.48204838
CTAATAC4403.6379788E-1214.3174793
Produced by FastQC (version 0.11.5)