FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2344559
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG2401.031367E-817.4995521
CGCACCT3700.017.02949766
GTATTAG10900.015.7335421
CCGCACC4151.8189894E-1215.18292465
CGGGCGA1859.0295485E-515.1373362
TCCTAGC4009.094947E-1214.8752548
CCCCTAT3707.039489E-1014.1888261
TCCGCAC4202.1827873E-1114.16872164
TCCTTGC12500.013.7202358
ACGCGCG1800.001260198813.61076238
TATACCG6250.013.4396565
GCGGGCG2354.5635825E-513.406261
TAGGTAG5300.013.2072095
TATACAG16200.013.1786765
CCTTGCG8000.013.1252249
CGCGGCC1600.00910001913.12494413
TAGCCTG5400.012.9626315
CCTATAC8850.012.6550433
TCCTATA11000.012.4087732
CCCTATA8050.012.1736012
Produced by FastQC (version 0.11.5)