FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858010
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG2203.4997356E-919.090021
CCCTAAG1150.001008049918.2600171
GACCGTG1000.00968777817.5002047
CTTAGAC2207.269955E-817.4991863
GTATATA7400.016.5532821
ACTGCTC2552.1735104E-816.470788
CCCTTGC2009.956562E-615.7501838
TCCTAGA2251.762457E-615.5548312
TTAGGCC1859.033678E-515.1344293
AGGCCGT1859.033678E-515.1344296
TATATAG6750.015.0363365
CCCTACA1901.1338314E-414.73615552
GTTAGAC2151.9751535E-514.650483
ACCGTGT1450.004738237714.4829278
GTCCCTA1450.00474007214.4820831
CCTACAC1708.1750104E-414.4110943
CGTATAC2951.3009594E-714.2366243
TACACCG2001.7536056E-413.9993485
GTATTAG3555.971742E-913.8021741
CCGTCCA1800.001258705313.611279
Produced by FastQC (version 0.11.5)