FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662410
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3250.019.38706464
GCCCGCC1653.3978657E-516.96928816
CCGCACC3900.016.15588865
ACTATGC7200.015.5556498
GTATATA18800.015.45175651
CTAATAC8850.015.4238223
CGCACCT4100.015.36779566
TAGGGGG1400.003754710815.000095
CCGACCG1400.003755462114.99963918
TACTATG9400.014.8937077
TATACTG10700.014.3926115
TACACAG5600.014.3750865
GGCCCGC1951.4152375E-414.35862915
GTATTAG7100.014.295431
CATATAC9450.014.074163
CTATGCA7100.013.80248459
CCGGCCC2052.1637039E-413.65820913
TCTACAC7300.013.4247393
AGTGTGC3405.0555172E-813.3824338
ACCGCGT2102.6536005E-413.33301338
Produced by FastQC (version 0.11.5)