FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065391
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG1352.3165921E-723.33316454
CGCGTAG800.002667972121.87484233
TATACTG12850.017.9765225
CCTCCTA4350.017.7010211
CCTTCTA8950.017.206581
CCTTGCG5900.017.2032649
TAGGTAG4150.016.8673485
GTATTAG8600.015.4650041
CTATACT9850.015.2790784
GCGCGCC1400.003755452814.9998928
TCTATAC10350.014.8791193
AACGCGC2151.977086E-514.65105652
TCCTTGC11800.014.5337928
GTACTAG5300.014.5281951
CAGGGGG1450.00474182714.48265521
CGCGGGA2202.4561594E-514.31807856
GTATATA23100.014.242321
GCTATAC5200.014.1345123
GCGCGGG2505.268581E-613.99989855
ACGCGCG2052.163795E-413.65843853
Produced by FastQC (version 0.11.5)