FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181711
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG7350.018.0949069
TAGGTAG4750.016.9470585
GACGCGC2602.7695933E-816.15318138
CGCGCAT3009.758878E-915.16604140
GTATTAG7850.015.158611
TCCTTGC12600.015.0000688
CGTCCGG2901.05626896E-714.48415332
ACGCGCA2901.0580516E-714.482161539
GTCTATA6300.014.4438481
ACCCTTG8100.014.2593257
ATCCTTG16500.014.2121877
CCTATAC7550.013.9070293
CCTCCTA3051.9589606E-713.7699241
TAGACTG6000.013.4164215
ATCCTAG4103.270543E-912.8043511
GTCCGGC3305.098191E-712.72849833
TCCTAAG4701.4370016E-1012.6593422
TATACTG11900.012.6468275
CCTACAC4751.72804E-1012.5260863
TCCTATA9850.012.436322
Produced by FastQC (version 0.11.5)