FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP2-F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP2-F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655126
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGC952.7824644E-422.10486222
CGACGGG1702.1212927E-618.52907649
TCCGCAC2751.6007107E-1017.8205564
TAGGTAG3700.017.0267185
GTATATA18950.016.622391
CGCACCT3401.0913936E-1116.47277866
CCGCACC3151.1059456E-915.55762465
CGGGCGG2051.2599259E-515.36557652
ACAGGTC3009.749783E-915.1663918
GTAGACC3553.783498E-1014.7884644
GTATTAG8050.014.7823411
ATAGGAC4051.0913936E-1114.6910923
ACTATGC5750.014.6084318
CGGGGGC1450.00474164314.48249617
TAATACT12150.014.403034
CCCTAGA2505.267657E-613.9997461
TAGGACA4801.8189894E-1213.8539144
CTGTCCG2303.732756E-513.6954039
GTATATG11250.013.6886411
TCTAAGC4102.237357E-1013.6582893
Produced by FastQC (version 0.11.5)