FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP2-E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP2-E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964781
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG5600.016.2498345
GTATATA18150.014.8479561
TATACTG12500.014.5598525
CCTACAC3403.261448E-914.4116183
CTAATAC10000.014.3498543
GTCTAGA5200.014.1341121
CATATAC12800.013.6717373
ACCGTGC1800.001259440913.6116668
GGCGCCG1800.001260166813.61062625
TATAAGG8250.013.5756192
GTACTAG4901.8189894E-1213.5709461
ATACAGC6300.013.3335386
GGGTGTC3951.87174E-913.291687
ACTGTGC4052.7193892E-912.96349058
CCCGTCC1900.001892633912.8952638
CTAGACT3002.4398378E-612.8332034
TATCCCG4103.268724E-912.8047485
TCATATA17350.012.5070782
GTATAAG8700.012.4708191
ACTAGAC3701.5260048E-712.2971733
Produced by FastQC (version 0.11.5)