FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP2-E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP2-E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222243
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT2700.022.04226766
CCGCACC2600.021.54357365
CCGAGGC1154.1200437E-521.30417313
TCCGCAC3150.020.00474564
TAGACAG3350.017.761055
GTATTAG4900.016.4277651
TAATACT11350.016.035114
ACCGTGC1554.0543987E-415.8069698
TAGGTAG3602.7284841E-1115.55542855
GCGTATG2302.2173554E-615.2166441
CAGGTCG1400.003754305414.9998789
CTATGCA5450.014.7705219
CTAATAC7250.014.482643
GTATAGA5100.014.41105651
TACACAG4700.014.148825
CCCAGTG2556.4445376E-613.72874766
CCTAATA7650.013.7253782
ACTATGC5900.013.6445148
GACAGTC1800.001259040213.6115577
CTATACA6250.013.439894
Produced by FastQC (version 0.11.5)