FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP2-E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP2-E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705336
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCG950.00721163218.4212159
CTAGACT2851.2732926E-1118.4206754
CGCGCGG1503.160753E-416.33299854
CTATACT7300.015.8215934
TCCGCAC2906.217306E-915.69163464
CGCACCT3201.382432E-915.31443166
CCGCACC3201.382432E-915.31443165
CGACCCG1400.003755426314.99969321
CCTATAC5150.014.2715513
ACCGTGT2556.4633696E-613.7256118
GGCTCGC1800.001258510513.61282862
TCCTTGC10150.013.4483958
TATACTG8600.013.4299575
CCTGTAC4701.0913936E-1113.403983
TATTAGG7100.013.3095862
TAGACTG3951.873559E-913.2908665
CCCTATA3951.873559E-913.2908662
CCTAGAC3456.122718E-813.1881353
GTATTAG7450.013.1540941
ATCCTAG4002.2610038E-913.1247311
Produced by FastQC (version 0.11.5)