FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP2-D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP2-D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495950
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG800.00266648421.87646131
GCTCGCG1551.9824003E-518.06753561
CAGCGTG2357.939889E-917.8723419
CGCACCT2207.262861E-817.50292666
GTATTAG7000.017.4994161
TATACAG11900.016.4700375
CCGCACC2151.0960084E-616.2817965
GGCTAGC1755.624284E-515.99946652
CATATAC12100.015.6193123
CTAATAC7850.015.158733
CTCGCGT1859.024369E-515.13766562
GGCGTCG1859.03209E-515.136147532
AGGACAG2553.9047518E-715.0975345
TATACGG6350.014.88139255
GCGACGT2604.8595757E-714.808681531
CCTATAC5200.014.8071983
ATACGGT5950.014.7053916
GTATAGC4051.0913936E-1114.6908671
TATACTG11300.014.5570355
CCGGCCC1450.00474078914.482758513
Produced by FastQC (version 0.11.5)