FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP2-D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP2-D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857494
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT4700.019.36678766
CCGCACC5450.017.34400265
TCCGCAC5300.016.5137764
TATACGG10600.016.1786795
CTAATAC13900.015.8627543
GTATTAG13050.014.7501821
CGGTCGG2656.0404636E-714.52779369
GTATATA30700.014.1360651
GTATAGA14700.014.0468821
GTCTCGC2303.735209E-513.69517369
GTGTATA11900.013.5287021
ACTATGC13900.013.3455578
TATACAG17650.013.2856545
TACACCG5650.013.0083945
TACACAG10250.012.9751565
TAGACTG5203.6379788E-1212.7880135
ACACCGT8800.012.7268276
TAATACT22700.012.642734
ATACGGT11400.012.5872796
TAGGACC5850.012.5636634
Produced by FastQC (version 0.11.5)