FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP1-F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP1-F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262547
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13300.015.7888941
GTATAGA16900.015.7390651
GTGTAAG10800.015.5549841
CCGCACC5350.015.04894465
TCCGCAC5550.013.87591664
GTCCTAC4555.456968E-1213.8456451
TATACGG12650.013.8336955
GTATATA39050.013.0853071
TACACCG6750.012.9626855
CTAATAC14650.012.9007473
CTATACT17350.012.7086614
TATACCG11350.012.64295
GTATTAA19850.012.5184311
CGCACCT5651.8189894E-1212.39120366
ACTATGC13000.012.3852998
CCGTCCG2852.032462E-512.2811919
GTCTTAG9150.012.2399861
TAGACAG11300.012.0793885
CCTAATA18400.011.98343852
CATATAC22200.011.9817253
Produced by FastQC (version 0.11.5)