FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP1-E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP1-E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905620
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG9350.016.8445535
GTATTAG16600.014.7585261
GTATAGA17900.014.273251
TATACCG11500.013.0866725
GTATAGG13300.012.8942911
TCTATAC17250.012.1736493
TATTAGG17350.012.1034822
GTATAAG15950.012.0685481
GTATAAA29500.011.7453571
GTGTAAG11350.011.7176561
GTATATA36400.011.6342131
TATATAC41300.011.5251733
TATACTG22800.011.5129075
GTATTAA22200.011.5086121
GTCCTAA10100.011.4352481
TATACAG20350.011.3511045
GTGTAGA15150.011.319741
ACAGCGC4954.3964974E-911.313038
ACTATGC16450.011.2764948
GACGGAC11850.011.22324170
Produced by FastQC (version 0.11.5)