FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP1-C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP1-C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3862820
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC7400.014.19168865
CGCACCT7300.013.90655766
TATACAG23950.013.8826595
GTATAGA18150.013.6910341
GTATAGG12650.013.5568911
CTAATAC17200.013.4298143
TCCGCAC7700.013.18413864
GTATTAT34400.013.1245921
TATACTG23650.013.0228515
TAGACTG7600.012.8943375
GTATTAG13850.012.8876861
TATATAC40500.012.8761433
ACCGCGC3356.129285E-712.5372498
CCGGACG3356.129285E-712.53724912
GTATATA38300.012.4278141
GCGGGGC3103.5258345E-612.41896826
TCTAGAC9050.012.3753063
TAGACCG9150.012.2400585
TACACTG12300.012.2353935
CAGTGCG3751.8188621E-712.133279
Produced by FastQC (version 0.11.5)