FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP1-B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP1-B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525708
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19350.017.7256471
TAGGTAG10550.013.601555
TATACTG26650.013.5268615
GTGTAAG11850.012.4047481
TATTAGG21000.012.3330192
TATACAG26550.012.2595765
CTTACAC12900.012.2089923
CCCTATA12700.012.1256752
GTATCCT16850.011.6317511
TACACTG14800.011.58754355
TAGGACA11850.011.5186954
TCCTTGC22900.011.4628458
CTAGGGT5501.6189006E-1011.4542544
CTATACT20000.011.3747114
TAGACTG10900.011.2382475
GTATAGA20300.011.2066121
ACTGTGC10950.011.1871778
GTATATA44700.011.1182851
TGCTAGA20900.011.0523512
GTATAAG19250.010.9088131
Produced by FastQC (version 0.11.5)