FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP1-A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP1-A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441314
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCCCG350.001255348139.997281
CTCCTAT1455.9299055E-1026.549921
TCCTATG1251.0977237E-725.1982882
TCATAGG700.001225843224.9982993
CCTATGT1001.3969608E-524.4983333
GTATTAA1506.371174E-720.9985711
GTTCCCT1506.371174E-720.9985711
TGGGCCG850.00378861120.5868365
CTAGACT900.00527388819.4431234
GTGTAAC1107.47056E-419.0896111
GTACTCT1301.0563111E-418.8448731
CCTATAC1301.0563111E-418.8448733
GATGGGA950.00719714918.42397533
CATGTAG950.00719714918.42397510
CCCCTAT950.00720656718.41981
GTCCTAT950.00720656718.41981
TTAACCT950.00720656718.41984
TCCGCAC1150.001005606518.26376764
TAGACCG1150.001007138318.2596285
GATGCTT1351.4094559E-418.1489726
Produced by FastQC (version 0.11.5)