FastQCFastQC Report
Tue 23 Aug 2016
HFWWMBGXY_n02_grads_set02_rna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWMBGXY_n02_grads_set02_rna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97139224
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2873480.29581047507647373No Hit
TTGAGTCCGTTAGAAGATTCTATTCAATTACATTCCATGACGATTCCGTT1215810.1251615928082769No Hit
TTGAGTTCTGGAATTGAATGTAATAGAATGGAGTGGAGTGGACTCGAATA1201110.12364830091704253No Hit
TTGAGTCCATTCCAATTCATTCGATTCCAATACATTGCATAACATTTGAA1153290.11872546974433314No Hit
TTGAGTATGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAAT1031980.10623720856571801No Hit
TTGAGTGGAACGGAATGGACTCGAATGGAGTGGAGTCAAATGGCCTCGAA1000650.10301194088188309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA22769300.0139.841431
TTGAGTT25176600.0138.079161
TTGAGTC12628000.0131.676761
TTGAGTG8358350.0126.561781
TGAGTAT13037950.0113.227732
GAGTATC4549050.0112.296133
TGAGTAC6165800.0111.954792
TGAGTTC9415600.0111.567542
GAGTACT3337350.0110.570433
TGAGTTA10055150.0110.48292
ACTAACG1400550.0108.308577
GAGTTAC3284850.0107.35973
TGAGTAG7196500.0103.999232
TGAGTCT4827700.0103.712912
TGAGTCC8351550.0103.3581542
GTGACCG633000.0102.8477255
GAGTAGC2865100.0102.717063
TGAGTTT7485350.0101.18592
GAGTTCC2259750.0101.0868153
GTTTGCG1074150.0100.202085