Basic Statistics
Measure | Value |
---|---|
Filename | HFWWMBGXY_n02_grads_set02_rna_digest_nextseq.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 97139224 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 287348 | 0.29581047507647373 | No Hit |
TTGAGTCCGTTAGAAGATTCTATTCAATTACATTCCATGACGATTCCGTT | 121581 | 0.1251615928082769 | No Hit |
TTGAGTTCTGGAATTGAATGTAATAGAATGGAGTGGAGTGGACTCGAATA | 120111 | 0.12364830091704253 | No Hit |
TTGAGTCCATTCCAATTCATTCGATTCCAATACATTGCATAACATTTGAA | 115329 | 0.11872546974433314 | No Hit |
TTGAGTATGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAAT | 103198 | 0.10623720856571801 | No Hit |
TTGAGTGGAACGGAATGGACTCGAATGGAGTGGAGTCAAATGGCCTCGAA | 100065 | 0.10301194088188309 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTGAGTA | 2276930 | 0.0 | 139.84143 | 1 |
TTGAGTT | 2517660 | 0.0 | 138.07916 | 1 |
TTGAGTC | 1262800 | 0.0 | 131.67676 | 1 |
TTGAGTG | 835835 | 0.0 | 126.56178 | 1 |
TGAGTAT | 1303795 | 0.0 | 113.22773 | 2 |
GAGTATC | 454905 | 0.0 | 112.29613 | 3 |
TGAGTAC | 616580 | 0.0 | 111.95479 | 2 |
TGAGTTC | 941560 | 0.0 | 111.56754 | 2 |
GAGTACT | 333735 | 0.0 | 110.57043 | 3 |
TGAGTTA | 1005515 | 0.0 | 110.4829 | 2 |
ACTAACG | 140055 | 0.0 | 108.30857 | 7 |
GAGTTAC | 328485 | 0.0 | 107.3597 | 3 |
TGAGTAG | 719650 | 0.0 | 103.99923 | 2 |
TGAGTCT | 482770 | 0.0 | 103.71291 | 2 |
TGAGTCC | 835155 | 0.0 | 103.358154 | 2 |
GTGACCG | 63300 | 0.0 | 102.847725 | 5 |
GAGTAGC | 286510 | 0.0 | 102.71706 | 3 |
TGAGTTT | 748535 | 0.0 | 101.1859 | 2 |
GAGTTCC | 225975 | 0.0 | 101.086815 | 3 |
GTTTGCG | 107415 | 0.0 | 100.20208 | 5 |