FastQCFastQC Report
Tue 23 Aug 2016
HFWWMBGXY_n02_grads_set02_dna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWMBGXY_n02_grads_set02_dna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103813786
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGA15473201.490476418998918No Hit
TTGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCAT6888250.663519775687595No Hit
TTGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTT6255110.6025317292637801No Hit
TTGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGAC6157940.5931717007219061No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCTTTCC5297030.510243408327291No Hit
TGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGAA4562380.4394772771315748No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTACATTCC4190550.40366026146084294No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTACTTTCC3473180.33455864907961264No Hit
TCAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCA3402650.32776475371007086No Hit
TTGAGTGACTGCCCATGTTATGTTTTCGTTGATGCTTATAATTCTGCAAT3339220.3216547752145365No Hit
TTGAGTGCCTGACGGAATGCGTATTGATACGTCTCACATGCACGGGATGG3142390.3026948655932845No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCATTCC2921990.28146454460296827No Hit
TTGAGCTTCACTTTCTATAATGTGTTATGAAAGCAGTAGGTATTTTTCAT2485670.2394354445372024No Hit
CAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCAG2414860.23261457779798148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2246880.21643368251688652No Hit
TTGAGTTCTGTAATATTTTGGATTTTCTACAACTTCCCCATTACTAGTAA2207940.21268273560507656No Hit
TGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGACT2063070.1987279415857158No Hit
TGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTTT2052020.19766353574659148No Hit
TGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCATT2046480.1971298879322251No Hit
TTGAGTCCGTTATTCGAGGATGATTCTATTCGAGTCCATTCAATGATTCC1818760.17519445827743918No Hit
TTGAGTATCATGTGCTTGAAATTGGCTCTTTTGCAGTTTGGTGTCATATT1758120.16935323021549373No Hit
TTGAGTGGAATCATCAAATGGATTCGAATGGAATCATCAAATGGACTCGA1547750.14908906221761337No Hit
TTGAGTCCCTTCCATTCCAGTCCATTATATTCGAGTCCATTCCATTCAAT1492570.14377377586441167No Hit
TGGAGTATTGAATCCGCCATGCAGAATCAGTTTAGCAATTATACAGCATA1470330.14163147850132352No Hit
TTGAGGACAGGACTTCTCTGTTCTTTATCACTGCAGTGTCTCCAGAGCTT1367400.13171661035462093No Hit
TGAGTGACTGCCCATGTTATGTTTTCGTTGATGCTTATAATTCTGCAATT1345320.12958972520277798No Hit
TTGAGTGGTTTCGTGATCAGAATTTGCAGAAATCTGTTGCTGTTTCTCGT1330380.12815060997775382No Hit
TGAGTGCCTGACGGAATGCGTATTGATACGTCTCACATGCACGGGATGGA1223800.1178841507620192No Hit
TTGAGTCCGTTTTATTCCAGTCCACTGCATTCTGGTCCATTCCATTTGAT1155590.11131373245553342No Hit
TGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCTTTCCG1106020.1065388367591179No Hit
TTGAGTTCTGGAATTGGATGGAATGGAATCAACTCGATTGCAATCGAATG1081640.10419040107062466No Hit
TTGAGTCCATTCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT1077830.10382339779034742No Hit
TTGAGTTACTAAACATCGCTTTTAACTTATTTAAAGTTGTCTCCAATTGG1070750.10314140744274562No Hit
TTGAGTACCAAAAACATTCTTTTGAAACATTATTCCTGGCCGTTCACCGC1062680.10236405403806388No Hit
TTGAGTGTGAAAGTTGGGTAACGGCCGTGCCATAGAAGAGAACGACAAGT1061640.10226387466496985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTT23197400.0138.303471
TTGAGTA20055600.0137.893891
TTGAGTC10804900.0130.239991
TTGAGTG10373150.0114.510671
GCCGCCT956450.0112.372771
CCGCCTT979100.0109.744952
AGTATCG3206650.0109.044214
GAGTATC7708800.0107.024043
TGAGTAT12358950.0106.2429662
CGCCTTA1015300.0105.7880863
TGAGTAC6313550.0102.813562
GAGTTAC4211900.0102.39043
TGAGTTA10198550.0102.2215652
GAGTACT3955900.0101.836423
GTATCGA3000000.0101.793565
TGAGTTC10618250.0101.136142
AGTTACG1890500.0100.628864
TCCGCAT1105500.099.7439657
TATCGAG3042950.099.447476
TAGTTCG1245600.098.4913566
Produced by FastQC (version 0.11.2)