FastQCFastQC Report
Tue 23 Aug 2016
HFWWMBGXY_n01_grads_set02_rna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWMBGXY_n01_grads_set02_rna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97139224
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT4281650.44077457320433194TruSeq Adapter, Index 19 (97% over 38bp)
CCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATCGC1705800.17560362639915675No Hit
TTGAGTTCTGGAATTGAATGTAATAGAATGGAGTGGAGTGGACTCGAATA1080770.11125989641424354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA16065300.0136.842931
TTGAGTT18213250.0134.759751
TTGAGTC9124200.0125.017651
GTGAGTA3967450.0120.039061
TTGAGTG6261300.0119.5385741
GTGAGTC1992650.0114.872881
TGAGTAT11191600.0111.082262
GAGTATC3953400.0110.18333
TGAGTTC8232900.0108.99122
TGAGTAC5395850.0108.091842
GAGTACT2884050.0107.715273
TGAGTTA8776400.0107.188312
ACTAACG1254150.0106.733387
CGCCTTA938250.0105.6005863
CCGCCTT942800.0105.112292
GAGTTAC2915700.0103.833773
TGAGTCT4074950.0101.697682
GTTTGCG986750.099.307135
GCCTTAC1060350.099.1886754
TGAGTAG6344150.098.692092
Produced by FastQC (version 0.11.2)