FastQCFastQC Report
Tue 23 Aug 2016
HFWWMBGXY_n01_grads_set02_dna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWMBGXY_n01_grads_set02_dna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103813786
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGA14238671.371558686820265No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCTTTCC11347631.0930754418300475No Hit
TGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGAA5626800.5420089389669307No Hit
TTGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTT5002770.4818984253208914No Hit
TTGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCAT4667290.44958287139243724No Hit
CAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCAG4230330.4074921224816904No Hit
TCAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCA3200580.3083000941705372No Hit
TGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCTTTCCG3111470.2997164557701421No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2544910.24514181575075203TruSeq Adapter, Index 3 (97% over 36bp)
TTGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGAC2412940.2324296312630386No Hit
TGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTTT2160590.20812168433968875No Hit
TGGAGTATTGAATCCGCCATGCAGAATCAGTTTAGCAATTATACAGCATA2139920.20613061929944448No Hit
GTGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGAC1844040.17762958765418688No Hit
GTGAGTGACTGCCCATGTTATGTTTTCGTTGATGCTTATAATTCTGCAAT1735520.16717625537710376No Hit
TGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCATT1732550.16689016620586403No Hit
GCAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCA1677670.16160377774874715No Hit
TCAAGAATTTAGAGACAGCATACAAAAACTTTTTTTCTGACTTGAAAAAG1542440.148577569456912No Hit
TTGAGTGGAATCATCAAATGGATTCGAATGGAATCATCAAATGGACTCGA1448410.13952000556072583No Hit
GTGAGTGCCTGACGGAATGCGTATTGATACGTCTCACATGCACGGGATGG1409350.1357574994904819No Hit
TTGAGCTTCACTTTCTATAATGTGTTATGAAAGCAGTAGGTATTTTTCAT1398560.13471813849463113No Hit
TTGAGTCCGTTATTCGAGGATGATTCTATTCGAGTCCATTCAATGATTCC1378440.13278005293054237No Hit
TTGAGTGACTGCCCATGTTATGTTTTCGTTGATGCTTATAATTCTGCAAT1231910.11866535721951224No Hit
TTGAGTGCCTGACGGAATGCGTATTGATACGTCTCACATGCACGGGATGG1117330.1076282874415157No Hit
GTGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCAT1088140.10481652215246248No Hit
TCGAGTGCAATGGAATCGAATGGAATGTAATAGTATGGAATGAAATCCAA1062320.1023293765627621No Hit
TTCAGGTGTTTCAGGGGTCATGGGAATCATCATCATTTACAGAAGTCTAC1059530.10206062612917324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTT16206700.0134.285191
TTGAGTA14761650.0134.273331
GTGAGTA2608700.0122.807681
TTGAGTC7923800.0122.729231
GCCGCCT1383900.0121.34681
CCGCCTT1409400.0119.253972
CGCCTTA1454050.0115.7047353
GCCTTAC1635550.0109.015274
GTGAGTT4498350.0105.971911
AGTATCG2777400.0104.868364
TGAGTAT10919500.0102.112772
GTGAGTC1518950.0101.855471
TTGAGTG7739750.0101.7928541
GAGTATC6881400.0101.6032943
TGAGTTC9060550.0100.269282
TGAGTAC5427050.099.733342
GAGTTAC3566350.099.564233
TGAGTTA8596150.099.145692
AGTTACG1654950.099.1045154
GAGTACT3394450.098.513923
Produced by FastQC (version 0.11.2)