FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056525
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3604412.564225510928199No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT253000.17998758583647095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC71950.039.0335121
ATCTAAG85150.032.9259535
CGCTAGG23850.022.50920546
TGCCTAG18350.022.28519666
GTGATCG23300.021.23169352
CATATCT133250.020.8093382
GCCTAGT18550.020.7226867
GAGAGAG28350.020.63445738
CTAAGTA136750.020.2981917
GGAGAGA29900.020.14472637
GCTAGGT25450.020.13425347
TCAACTG30800.019.6384623
CTAGGTG25900.019.37845448
CTAGTCT20500.019.26269569
AAGTATA152950.018.7720859
TAAGTAT151400.018.617528
TATAAGC149750.018.56365412
TGGCTCA139750.018.46131342
AGAGTCG31950.018.43059541
AGTCGCT30000.018.34534343
Produced by FastQC (version 0.11.5)