FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n02_Sorb_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n02_Sorb_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5705177
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT282850.49577778217923824No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT99020.17356166162767606No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA94430.16551633717937234No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC93180.16332534468255763No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT88540.1551923805343813No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA83790.14686660904648532No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC82770.1450787591690845No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA81250.1424145122929578No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC76360.13384334964541855No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT71990.12618363987655423No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA68490.12004886088547297No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC68170.1194879668062884No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC67920.11904976830692543No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA66760.11701652726988138No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT65250.11436980833372917No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA61710.10816491758274985No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA61550.10788447054315756No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG61440.10769166320343787No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC61260.10737616028389653No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT57890.10146924451248401No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC57530.10083823867340137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC50850.044.907061
ATCTAAG60800.037.710595
CATATCT61550.037.2602272
ATATCTA62400.036.7507483
ACACGTA6950.034.24073815
CGCTAGG8900.030.28677446
CACGTAA8250.028.42153416
GCCTAGT8350.025.9844167
GTGATCG9850.025.94275552
TGCCTAG8550.025.78611266
GCTAGGT10300.025.4910547
GAGTCGC10750.024.74551642
CTAAGTA100300.023.1337937
AACACGT10450.022.43746414
CTAGGTG11900.022.35806348
CCCCACG11600.022.325857
GGAGAGA12200.022.08883337
GAGAGAG11900.022.0584638
CTAGTCT10000.022.04751269
CCCACGC11950.021.6719558
Produced by FastQC (version 0.11.5)