FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n02_Sorb_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n02_Sorb_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175776
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT299070.48426303026534645No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA143420.23222992543771018No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT138540.22432808443829572No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC115780.18747441617053467No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA110310.1786172296404533No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA99070.16041708766639207No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC95600.15479836056230017No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT95100.1539887457058028No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA94200.1525314389641075No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT90390.1463621737575974No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG90240.14611928930064821No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT89320.14462959796469302No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA88430.14318848352012767No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC82030.13282541335696113No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC76580.12400061142113963No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA74310.1203249599726415No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA71680.11606638582746524No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC71290.11543488623939728No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT69980.11331369531537414No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC67550.10937896711279685No Hit
CCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCA66690.10798642955962133No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT66210.10720919929738384No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT65560.10615669998393724No Hit
AAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAG64330.10416504743695368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC54700.045.06851
ATCTAAG63450.039.109685
TGCCTAG20050.039.09415466
CGCTAGG23100.038.4949746
GCTAGGT23650.038.04444547
ACACGTA8550.037.65821515
CATATCT66450.037.2465022
GAGTCGC25550.036.99183342
GCCTAGT20850.036.92072767
ATATCTA67000.036.834463
GTGATCG23600.036.49175652
GAGAGAG26500.036.45680638
CTAGTCT22100.035.467269
CGGTGGA27800.035.37639633
GGAGAGA28150.034.692137
CTAGGTG25950.034.40266448
GGTGATC25150.034.24359551
TCAACTG29650.033.4119453
CAAACGG29250.033.02148429
TAGTCTC22800.032.99782670
Produced by FastQC (version 0.11.5)