FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n02_SC_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n02_SC_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227735
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT442780.538155397566888No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA161290.19603207930250546No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT141070.1714566645619967No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA125780.15287317834130537No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT125190.15215609156104323No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA119350.14505814783777066No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC115970.14095009136779443No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT113240.137632045757429No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC112160.13631941232915254No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT103010.1251984902284772No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA99290.12067719730885838No Hit
CTTCAACTGCTTTCGTAAATGGCCTCGTCAAACGGTGGAGAGAGTCGCTA97470.1184651669019481No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC96980.11786962025393381No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT96170.11688514518272647No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA93250.1133361733210902No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC90450.10993304961815129No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG83790.10183847681044661No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA83760.10180201477077228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83340.10129154621533144No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG83330.10127939220210666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC80500.044.3688621
CGCTAGG28850.043.9272246
TGCCTAG26200.042.47368266
GTGATCG29500.041.77235852
GCCTAGT26850.041.57502767
GCTAGGT30350.041.06534647
GAGTCGC33100.040.18590542
GAGAGAG34200.039.09570338
ATCTAAG91700.038.7423365
CTAGTCT29000.038.61458269
GGTGATC32500.037.48717551
CGGTGGA34950.037.45122533
CTAGGTG33500.036.99541548
CATATCT96750.036.6569142
GGAGAGA36450.036.58547237
TAGTCTC28750.036.2736370
ATATCTA98900.036.0708853
CAAACGG39650.033.7159529
AGTCGCT39550.033.36749643
CGTCAAA41850.033.0308326
Produced by FastQC (version 0.11.5)