FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n02_SC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n02_SC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6653111
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT354420.5327131923697049No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA121010.18188483553032558No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT115690.17388857633669422No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA110680.1663582645772782No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA108390.16291626578904217No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT103200.15511540390653336No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC93000.13978423026460854No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC92560.1391228855192706No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT89840.13503457254809068No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA87000.13076589282818218No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT86520.13004442583326808No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC86480.1299843035836919No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT83190.12503924855605145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82380.1238217730021339No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT75270.11313504313996865No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC73870.1110307644048025No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT73690.11076021428170972No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA73550.1105497864081931No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG68800.10341026927102223No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT68650.10318481083511158No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC68630.1031547497103235No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA66950.10062961522812411No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA66910.10056949297854792No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA66580.10007348441954447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC63150.043.248661
TGCCTAG19600.043.02864566
GCCTAGT20150.041.5055867
CGCTAGG20950.041.4432446
GAGTCGC23000.039.8735142
GCTAGGT22200.039.58550647
CTAGTCT20800.039.19940669
GTGATCG21900.039.0060352
ATCTAAG70400.038.928775
CATATCT72550.037.491582
GAGAGAG25450.037.27031738
ATATCTA74600.036.742323
CGGTGGA25500.036.3712233
TAGTCTC21950.036.34883570
GGAGAGA26550.035.59352537
GGTGATC23450.035.38514751
CTAGGTG25850.033.9968348
TCAACTG29650.033.058033
AGTCGCT29450.031.26039543
TCTGCCT26100.031.1048364
Produced by FastQC (version 0.11.5)