FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n02_SC_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n02_SC_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618581
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT257110.5566861336847833No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA92050.19930363893152464No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA83070.1798604376539028No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT81340.1761146984322674No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC71750.15535074517476255No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT71170.15409494821028363No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT65960.14281442720177473No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA62270.13482496030707267No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT60300.1305595809622046No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA58110.12581786483770666No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC57620.12475693291943998No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA56600.12254846239570119No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC55190.11949557667170933No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC55030.11914914992288757No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC54800.11865116147145628No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC54070.11707058942995695No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG53400.11561992741926579No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA49440.10704586538592697No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA47970.10386306963112697No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC47850.10360324956951064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC46750.045.8380931
ATCTAAG54900.039.0861175
CATATCT54850.038.8698352
ATATCTA55550.038.632533
CGCTAGG10650.038.13036746
TGCCTAG9850.036.94835366
GCTAGGT11350.035.4729747
GCCTAGT10600.034.33259667
CTAGTCT10900.033.0662769
GAGTCGC12450.032.89445542
GAGAGAG12650.032.6493238
GTGATCG11500.032.26719752
GGAGAGA12850.031.59502437
GGTGATC12700.029.2199151
CTAGGTG13750.029.02638648
TAGTCTC11650.028.53493170
CGGTGGA14900.027.9506333
CCCCACG8050.027.8252147
AGTCGCT15100.027.585843
CCCACGC8150.027.4826098
Produced by FastQC (version 0.11.5)