FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056525
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1024290.7286936138199164No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT460460.3275774062223772No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA165080.11744012122484042No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT158760.11294398864584242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC92950.043.2046661
ATCTAAG112200.037.566795
CGCTAGG20000.030.4562646
ACACGTA13450.030.4258215
CTAGGTG19500.029.43147348
TGCCTAG16450.028.9417166
GCTAGGT21200.028.72690447
GTGATCG19500.028.16452652
GAGAGAG25550.027.40728838
CATATCT155200.027.0481932
GGAGAGA25900.027.03662937
GCCTAGT17900.026.79207467
CGGTGGA26350.025.63857333
ATATCTA167500.025.1038443
CTAGTCT19550.024.34754669
GGTGATC22050.024.11460351
CTAAGTA189750.022.9111257
AGAGTCG31550.022.52901541
AGTCGCT31300.021.92518843
AAGTATA207850.021.6563649
Produced by FastQC (version 0.11.5)