FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_Sorb_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_Sorb_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16652979
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT731710.439386850845125No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT537630.3228431381556417No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA433220.26014564721423117No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA415470.24948689360624307No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC341400.2050083651699795No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC337420.2026184023891461No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA302850.1818593538129124No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC274220.16466723461309835No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC265190.15924478136914724No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT262590.15768349915051236No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT261370.15695089749407598No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA255160.15322183496418268No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA242040.14534336469168668No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG238460.14319359917525867No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA237300.1424970271084831No Hit
GTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGC234360.14073157721510368No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT232030.13933242814994243No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT220880.13263692940464283No Hit
ATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGGCCCGG219490.13180224391083423No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT214080.12855357590975164No Hit
CTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAGAACCAAACGT211950.12727452547679308No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC211700.12712440218653973No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC208640.12528689311383867No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA197130.11837521683057428No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTA195530.11741442777295281No Hit
ATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCA193180.11600326884457128No Hit
TATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGGCCCGGT189600.11385350332814327No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG188650.11328303482518053No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA186510.11199797946061182No Hit
AAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAG183580.11023853449884252No Hit
AATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTAT179060.10752430541106188No Hit
CCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCA178920.10744023636852001No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT176260.10584292456022433No Hit
ATACAGGGCCCATTCGGGTCTTGTAATTGGAATGAGTACAATGTAAATAC175260.10524243139921091No Hit
GTACAATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGC174670.104888140434213No Hit
GAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC174050.10451583467438468No Hit
TCCTAATTCAGGGAGGTAGTGACAATAAATAACGATACAGGGCCCATTCG172350.10349499630066188No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT170420.102336044499906No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC168970.10146532941643654No Hit
TCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATC168230.10102096447728662No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT167630.10066066858067857No Hit
TTACTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAA167620.10065466364906843No Hit
ATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCTTC167020.10029436775246038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG35700.046.3814346
GAGTCGC40550.044.46542442
GCTAGGT37100.043.6832947
CTAGGTG36800.042.8914248
GCATATC138700.041.231861
TGCCTAG33350.040.51848266
GCCTAGT34350.040.25552767
GTGATCG35850.039.9211352
CGGTGGA47250.038.00625233
GGAGAGA47550.037.91996837
GAGAGAG48400.037.76072738
CTAGTCT37000.035.94970369
ATCTAAG166250.035.8067445
AGTCGCT51500.034.5348343
GGTGATC41100.034.4813751
ATATCTA171650.034.456223
CATATCT172100.034.203652
CAAACGG55550.032.3240629
CGTCAAA57150.032.1474826
ACACGTA14400.031.34821715
Produced by FastQC (version 0.11.5)