FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_Sorb_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_Sorb_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15405821
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT971430.6305603576725967No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT367150.23831900941858275No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA343720.2231104723338016No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC338220.2195403932059187No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC261790.16992927543426603No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA252940.16418469356485446No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA247460.16062759654289116No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA240420.15605789525920105No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT232130.15067681235553756No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC225980.14668481478526849No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT210340.1365328079561615No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT207430.13464391154486346No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC204080.1324694088033348No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT196510.12755568171277598No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA196050.12725709327662577No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC193730.12575116898995514No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG192340.12484891262854475No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA190480.12364157677802436No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA185120.1201623723915785No Hit
AAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAG184570.1198053644787902No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA179680.11663123958145431No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC179660.11661825747553473No Hit
TCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATC174920.11354149837259565No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT174730.1134181683663597No Hit
AATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTAT171750.11148383458434315No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC158720.10302599257774059No Hit
GTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTA155550.10096832878948808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC165600.045.75551
ACACGTA23050.038.7135415
CATATCT199500.038.6457632
ATCTAAG203700.037.9347235
ATATCTA204800.037.8336223
CACGTAA25800.034.72261416
AAACGCG10450.029.46777213
AACACGT31300.028.84480314
CTAAGTA339650.023.2938587
CATATGC84900.023.2076383
AACGCGT13300.023.1538514
CCCACGC26800.022.3271758
AAGTATA358900.022.181189
CGCTAGG17300.022.05690846
TAAGTAT362650.021.9323028
CCCCACG26850.021.8946237
GTCTCAA90900.021.51926412
AGTATAA373450.021.44822710
GCTTGTC92100.021.3905168
TCATATG93950.021.1956022
Produced by FastQC (version 0.11.5)