FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_Sorb_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_Sorb_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9781450
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT647340.6618037203073165No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA223430.22842216644771482No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT215250.22005939814649156No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC213080.21784091315704723No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA158490.16203119169448293No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA146020.14928257058002647No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC143030.14622576407383364No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT141860.1450296223974973No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA137660.1407357804824438No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT136850.13990768239882634No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT132560.13552182958559314No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC129720.1326183745763665No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA127990.1308497206446897No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA126000.12881525745160483No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC125790.12860056535585215No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT119640.1223131539802381No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG117250.11986975346191003No Hit
AAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAG114040.11658803142683344No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC113890.11643467992986725No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC112010.11451267450122427No Hit
TCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATC110700.11317340476105281No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC110240.11270312683702313No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA108370.11079134484151122No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA105070.10741761190825493No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA100870.10312376999320141No Hit
AATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTAT99180.10139600979404895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC111600.043.8440741
ACACGTA18400.041.07833515
CACGTAA19950.038.93939216
CATATCT133900.037.2984962
ATCTAAG137250.036.7445455
ATATCTA136500.036.613693
CGCTAGG14900.035.9471246
GAGTCGC17750.034.32283442
TGCCTAG14850.032.29665866
AACACGT23800.032.19862714
GCTAGGT17100.031.52271547
GCCTAGT15900.030.3837267
CTAGGTG18250.028.76549748
GTGATCG17350.028.43696252
GAGAGAG23400.026.48423638
CTAGTCT17450.025.67636369
AAACGCG7750.025.28449813
GGAGAGA24800.025.27087637
CGGTGGA24600.025.04448533
AACGCGT8300.023.60913814
Produced by FastQC (version 0.11.5)