FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_Sorb_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_Sorb_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175776
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT344270.5574522132927101No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA159900.258914831107864No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT158540.25671267869819114No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC120110.19448568082780204No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA113580.1839121108019462No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA106660.17270704118802235No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC102440.16587389179918444No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT99130.16051424144917173No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA97160.15732435891457203No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT96040.15551082163601787No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT93550.15147893965066092No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA90380.14634598146046748No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG89430.14480771323312244No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC85580.13857367883809257No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA82130.13298733632826062No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC81670.132242490660283No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC81650.13221010606602313No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT74840.12118315172052874No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA74560.12072976740089018No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT71570.11588827055903582No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC71100.11512723259392828No Hit
CCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCA70370.1139451949034421No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT68950.11164588871098952No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT68280.11056100480328303No Hit
TCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATC68110.11028573575207393No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAA65840.10661008430357577No Hit
AAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAG65830.10659389200644583No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA65300.1057357002585586No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG63290.10248104853543911No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT63240.10240008704978938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC60800.044.0947841
CGCTAGG20950.041.44046446
ATCTAAG68600.039.689795
GAGTCGC23700.039.5922642
GCTAGGT21850.039.2481247
GCCTAGT20150.039.09052767
GAGAGAG24400.039.03007538
GTGATCG19950.038.93894252
CGGTGGA24650.038.48564533
TGCCTAG20400.037.9259766
ATATCTA72000.037.7675553
GGAGAGA25500.037.48281537
CTAGGTG22950.037.361548
CATATCT73600.036.8992732
ACACGTA9400.036.48235715
CTAGTCT21250.036.23801869
GGTGATC22150.035.38766551
TCAACTG27800.034.493413
TAGTCTC21250.034.42305470
CAAACGG27650.033.7978229
Produced by FastQC (version 0.11.5)