FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_SC_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_SC_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227735
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT495630.6023893574598599No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA179030.21759329876326838No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT145520.1768652004470246No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT142900.17368084898213176No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA130690.15884079883467322No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC127610.15509736276144043No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA127390.1548299744704952No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC123820.15049099174924813No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT111240.13520124311247264No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT110310.13407091988256792No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA108960.13243012809722238No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT108370.13171304131696027No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC101050.12281630363641999No Hit
CTTCAACTGCTTTCGTAAATGGCCTCGTCAAACGGTGGAGAGAGTCGCTA98470.11968056822442628No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA97810.11887840335159068No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA93030.11306878503014499No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA92160.11201138587958898No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG87420.1062503836110424No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC87350.10616530551846894No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC86830.10553329683078028No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG85650.10409912327025604No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA82390.10013691495897718No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC82310.10003968285317892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG24800.048.55626346
GCTAGGT25900.045.4069747
GCATATC84700.045.207071
GCCTAGT22850.045.03930367
TGCCTAG22400.044.6950266
GAGTCGC30300.044.0242642
CTAGGTG27550.042.42902448
CTAGTCT24850.042.39711869
GAGAGAG31600.042.3223938
GTGATCG26500.041.86046652
GGAGAGA33350.040.3105537
ATCTAAG97550.039.9320765
CATATCT100950.038.6572042
CGGTGGA34550.038.60085333
ACACGTA11550.037.87249815
ATATCTA103600.037.6343773
AGTCGCT35150.036.754343
GGTGATC29900.036.63226351
TAGTCTC27500.035.50858770
CAAACGG37750.034.95442229
Produced by FastQC (version 0.11.5)