FastQCFastQC Report
Thu 20 Dec 2018
HFWWGAFXY_n01_SC_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWWGAFXY_n01_SC_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9509838
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT706890.7433249651571352No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA224900.2364919360350828No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT222770.23425215024693377No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC195120.2051769967059376No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA180870.19019251432043321No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT168440.17712183950977922No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA166800.1753973096071668No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT158250.1664066201758642No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA149150.1568375823016123No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC145960.15348316133250642No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC143740.15114873670823836No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC141530.14882482751020573No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT138610.1457543230494568No Hit
TAGACATGCATGGCTTAATCTTTGAGACAAGCATATCTAAGTATAAGCAA135390.14236835580164459No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC127150.13370364458364065No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC126900.13344075892775462No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA122410.1287193325480413No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA122070.12836180805603628No Hit
TCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATC119700.12586965203823663No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG117530.12358780454514576No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT116260.12225234541324469No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT113940.1198127665266222No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC113720.11958142714944249No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA113570.11942369575591089No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT112820.11863503878825277No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA112730.1185403999521338No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT111380.11712081741034916No Hit
AAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAG109960.11562762688491644No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA107240.11276743094887631No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA104420.10980208075048177No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA104340.10971795734059824No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT103740.10908703176647173No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA103630.10897136207788187No Hit
AATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTAT96540.10151592487695374No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT95480.10040128969599693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATC120200.048.4563031
CATATCT138400.042.384972
ATATCTA139600.042.246063
ATCTAAG140050.042.1346475
ACACGTA14400.038.63945415
CGCTAGG11700.037.9975746
GCCTAGT11300.033.4560167
CACGTAA16800.033.3278316
AAACGCG9000.033.05010213
TGCCTAG11450.033.0182466
GCTAGGT13650.032.56643347
AACACGT18800.030.1544414
AACGCGT10100.029.79690414
GAGTCGC15400.029.7803442
CTAGGTG15250.029.37606248
GTGATCG13900.028.69984852
GGAGAGA17300.027.3186937
GAGAGAG17500.026.80727638
CTAGTCT13550.026.09015569
CTAAGTA236750.025.4969737
Produced by FastQC (version 0.11.5)