FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24833229
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3439101.3848783015692403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT14850.09.0335221
CGGGGAT40300.08.916241
CGGGGTT42350.08.3715111
CGGGAAT28750.07.99887561
CTCGGAT33600.07.98498871
CGAATTG20400.07.96212053
GGGGAAT62100.07.8692641
CGCGGGT27400.07.6935661
CCGGGAT34500.07.3600761
CTCGGTT22850.07.3385011
TCGGATT22700.07.16903452
GATCGGA231250.07.129789494-95
CCCGTAT19853.6379788E-126.99943541
GGGAAAT102600.06.86428831
AGATCGG252600.06.809734394-95
TACCGTA15501.909757E-86.72661649
CGGGAAC41400.06.7120191
ATCGGAA230550.06.656194-95
CCCGGTT25300.06.6278561
CGGAAAT34950.06.57990461
Produced by FastQC (version 0.11.5)