FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm232RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm232RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33947211
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT114450.010.2633091
CGCGAAA16900.09.5674991
CGCGATT13301.0913936E-118.5815521
GCGTCGT256000.08.053225594-95
CCGGGAT94950.07.8634091
CGGGAAT59950.07.7739741
CGGGGTT159400.07.6077831
CCGGTAT56000.07.47310161
CGTCGTG247750.07.468164494-95
CGCGGGT82450.07.44055371
AGATCGG679300.07.26913894-95
GATCGGA621550.07.25287594-95
AGCGTCG304300.07.141824794-95
GGGGAAT183350.07.05495451
CGCATAT24400.07.01647421
AGAGCGT355600.06.979826594-95
GTCCGAT25900.06.97734261
CGGGATT82450.06.86376671
TCGGAAG565500.06.799927794-95
ATCGGAA632000.06.757135494-95
Produced by FastQC (version 0.11.5)