FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm231RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm231RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32679039
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT97250.010.5129831
CGCGGAT32900.07.9495761
CCGGGAT86600.07.90718171
CGGGATT65000.07.6816241
GCGTCGT268050.07.655776594-95
CGCGATT12006.723167E-87.5292111
AGCGTCG311900.07.50087994-95
CGCGAAA15352.5283953E-107.43498331
CGGGGTT141950.07.4034481
CGTCGTG260400.07.360780794-95
GTCGGAT35700.07.32607941
AGATCGG711700.07.158493594-95
GATCGGA652300.07.154948794-95
CGGGAAT63200.07.14798551
CGGGCAT109350.07.13185021
CGCGGTT30150.07.097451
CGGGTAT29800.07.0212351
GCCGATT33100.06.89589071
CCCGGAT85500.06.7853281
GAGCGTC369800.06.78247794-95
Produced by FastQC (version 0.11.5)