FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm230RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm230RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41051247
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA41250.013.1260049
CGCGGGT71400.010.7235021
CGGGGAT124250.010.0662651
CTACCGT56200.010.0568578
CCCGTAT59700.08.60316851
CGGGAAT95400.08.4744111
CGCGGAT47700.07.87621741
ACTACCG74100.07.7556047
CGTATCG46050.07.7365013
GGGGAAT194850.07.56606861
GCGTCGT319100.07.532700594-95
AGCGTCG369050.07.491430894-95
CGGGTCT140650.07.4301223
CCGGGAT110750.07.38573461
CGGGTAT40450.07.28923851
CTCGGAT107250.07.2720281
CGGGGTT125550.07.23478461
AGATCGG836750.07.184448794-95
GATCGGA748300.07.12586594-95
ATCGGAA712550.07.10338194-95
Produced by FastQC (version 0.11.5)