FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm229RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm229RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30325309
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT62250.011.6111841
TACCGTA29800.09.40378959
CGGGGAT103800.09.0706851
CCCGTAT44000.08.9701091
CGTATCG34450.07.99722963
CGGGAAT68200.07.7394641
GATCGGA532350.07.62930794-95
AGATCGG598600.07.626106794-95
GGGGAAT153700.07.5799181
CGGGTAT33450.07.5344581
CGCGAAT18950.07.52808141
CTCGGAT83050.07.44347951
CGGGTCT113700.07.4363593
GCGTCGT231700.07.401118894-95
GTCCGAT27300.07.31575061
CGGGGTT94400.07.30412631
CTCGTAT31300.07.1404541
CCGGGAT82700.07.1299821
CTCGGTT56300.07.09484961
AGCGTCG270850.06.953933794-95
Produced by FastQC (version 0.11.5)