FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm224RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm224RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34525370
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA33350.010.1115659
CGCGGGT67400.09.8782571
CGGGGAT116850.08.7502791
GGGGAAT173550.08.6043331
CGGGGTT113400.07.8841881
CCGGGAT99200.07.81426431
GATCGGA565350.07.76802894-95
CTACCGT44850.07.73064338
AGCGTCG278850.07.674416594-95
CGGGAAT82450.07.6713741
GCGTCGT239950.07.433792694-95
AGATCGG634150.07.355983394-95
ATCGGAA535700.07.262455594-95
CGACCTA22000.07.12446264
CGCCAAT62850.07.11270431
CGCGGTT42150.06.99529931
CGGGTAT36350.06.93399671
CTCGGTT68450.06.87818671
AGAGCGT344650.06.822572794-95
CGTCGTG237400.06.743268594-95
Produced by FastQC (version 0.11.5)